abstract：BACKGROUND:Conserved two-component system ColRS of Pseudomonas genus has been implicated in several unrelated phenotypes. For instance, deficiency of P. putida ColRS system results in lowered phenol tolerance, hindrance of transposition of Tn4652 and lysis of a subpopulation of glucose-grown bacteria. In order to disco...

journal_title：BMC molecular biology

authors： Kivistik PA,Kivi R,Kivisaar M,Hõrak R

更新日期：2009-05-16 00:00:00

abstract：BACKGROUND:Among the three functions of DNA, mammalian replication and transcription can be subject to epigenetic imprinting specified by the parental origin of chromosomes, and although there is suggestive indication that this is also true for meiotic recombination, no definitive evidence has yet been reported. RESUL...

journal_title：BMC molecular biology

authors： Ng SH,Madeira R,Parvanov ED,Petros LM,Petkov PM,Paigen K

更新日期：2009-05-13 00:00:00

abstract：BACKGROUND:The human adenovirus type 5 early region 1A (E1A) gene encodes proteins that are potent regulators of transcription. E1A does not bind DNA directly, but is recruited to target promoters by the interaction with sequence specific DNA binding proteins. In mammalian systems, E1A has been shown to contain two reg...

journal_title：BMC molecular biology

authors： Yousef AF,Brandl CJ,Mymryk JS

更新日期：2009-04-17 00:00:00

abstract：BACKGROUND:Nucleosomes are nucleoproteic complexes, formed of eight histone molecules and DNA, and they are responsible for the compaction of the eukaryotic genome. Their presence on DNA influences many cellular processes, such as transcription, DNA replication, and DNA repair. The evolutionarily conserved histone vari...

journal_title：BMC molecular biology

authors： Gervais AL,Gaudreau L

更新日期：2009-03-04 00:00:00

abstract：BACKGROUND:Duchenne Muscular Dystrophy (DMD) is an X-linked genetic disorder that results in the production of a dysfunctional form of the protein, dystrophin. The mdx5cv mouse is a model of DMD in which a point mutation in exon 10 of the dystrophin gene creates an artificial splice site. As a result, a 53 base pair de...

journal_title：BMC molecular biology

authors： Maguire K,Suzuki T,DiMatteo D,Parekh-Olmedo H,Kmiec E

更新日期：2009-02-23 00:00:00

abstract：BACKGROUND:Research using the zebrafish model has experienced a rapid growth in recent years. Although real-time reverse transcription PCR (QPCR), normalized to an internal reference ("housekeeping") gene, is a frequently used method for quantifying gene expression changes in zebrafish, many commonly used housekeeping ...

journal_title：BMC molecular biology

authors： McCurley AT,Callard GV

更新日期：2008-11-12 00:00:00

abstract：BACKGROUND:The challenge of determining amplification efficiency has long been a predominant aspect of implementing real-time qPCR, playing a critical role in the accuracy and reliability that can be achieved. Based upon analysis of amplification profile position, standard curves are currently the gold standard for amp...

journal_title：BMC molecular biology

authors： Rutledge RG,Stewart D

更新日期：2008-10-30 00:00:00

abstract：BACKGROUND:SRY is the pivotal gene initiating male sex determination in most mammals, but how its expression is regulated is still not understood. In this study we derived novel SRY 5' flanking genomic sequence data from bovine and caprine genomic BAC clones. RESULTS:We identified four intervals of high homology upstr...

journal_title：BMC molecular biology

authors： Ross DG,Bowles J,Koopman P,Lehnert S

更新日期：2008-10-14 00:00:00

abstract：BACKGROUND:CCAAT/Enhancer Binding Proteindelta (C/EBPdelta) is a member of the highly conserved C/EBP family of leucine zipper (bZIP) proteins. C/EBPdelta is highly expressed in G0 growth arrested mammary epithelial cells (MECs) and "loss of function" alterations in C/EBPdelta have been associated with impaired contact...

journal_title：BMC molecular biology

authors： Zhang Y,Liu T,Yan P,Huang T,Dewille J

更新日期：2008-10-01 00:00:00

abstract：BACKGROUND:The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding sequences with fluor...

journal_title：BMC molecular biology

authors： Frandsen RJ,Andersson JA,Kristensen MB,Giese H

更新日期：2008-08-01 00:00:00

abstract：BACKGROUND:Traumatic brain injury models are widely studied, especially through gene expression, either to further understand implied biological mechanisms or to assess the efficiency of potential therapies. A large number of biological pathways are affected in brain trauma models, whose elucidation might greatly benef...

journal_title：BMC molecular biology

authors： Rhinn H,Marchand-Leroux C,Croci N,Plotkine M,Scherman D,Escriou V

更新日期：2008-07-08 00:00:00

abstract：BACKGROUND:Myocyte stress 1 (MS1) is a striated muscle actin binding protein required for the muscle specific activity of the evolutionary ancient myocardin related transcription factor (MRTF)/serum response factor (SRF) transcriptional pathway. To date, little is known about the molecular mechanisms that govern skelet...

journal_title：BMC molecular biology

authors： Ounzain S,Dacwag CS,Samani NJ,Imbalzano AN,Chong NW

更新日期：2008-05-19 00:00:00

abstract：BACKGROUND:Senescence marker protein-30 (SMP30), whose expression declines during aging in rat liver, has been proposed as an important aging marker. Besides apoptosis, SMP30 also protects cells against various other injuries by enhancement of membrane calcium-pump activity. The mechanism of this differential gene expr...

journal_title：BMC molecular biology

authors： Rath B,Pandey RS,Debata PR,Maruyama N,Supakar PC

更新日期：2008-04-29 00:00:00

abstract：BACKGROUND:GFG/NUDT is a nudix hydrolase originally identified as the product of the fibroblast growth factor-2 antisense (FGF-AS) gene. While the FGF-AS RNA has been implicated as an antisense regulator of FGF-2 expression, the expression and function of the encoded GFG protein is largely unknown. Alternative splicing...

journal_title：BMC molecular biology

authors： Zhang SC,MacDonald KA,Baguma-Nibasheka M,Geldenhuys L,Casson AG,Murphy PR

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:Wnt signaling is mediated through 1) the beta-catenin dependent canonical pathway and, 2) the beta-catenin independent pathways. Multiple receptors, including Fzds, Lrps, Ror2 and Ryk, are involved in Wnt signaling. Ror2 is a single-span transmembrane receptor-tyrosine kinase (RTK). The functions of Ror2 in ...

journal_title：BMC molecular biology

authors： Li C,Chen H,Hu L,Xing Y,Sasaki T,Villosis MF,Li J,Nishita M,Minami Y,Minoo P

更新日期：2008-01-23 00:00:00

abstract：BACKGROUND:In spite of large intergenic spaces in plant and animal genomes, 7% to 30% of genes in the genomes encode overlapping cis-natural antisense transcripts (cis-NATs). The widespread occurrence of cis-NATs suggests an evolutionary advantage for this type of genomic arrangement. Experimental evidence for the regu...

journal_title：BMC molecular biology

authors： Jin H,Vacic V,Girke T,Lonardi S,Zhu JK

更新日期：2008-01-14 00:00:00

abstract：BACKGROUND:Although much is known about molecular mechanisms that prevent re-initiation of DNA replication on newly replicated DNA during a single cell cycle, knowledge is sparse regarding the regions that are most susceptible to re-replication when those mechanisms are bypassed and regarding the extents to which check...

journal_title：BMC molecular biology

authors： Mickle KL,Oliva A,Huberman JA,Leatherwood J

更新日期：2007-12-21 00:00:00

abstract：BACKGROUND:In budding yeast, the replication checkpoint slows progress through S phase by inhibiting replication origin firing. In mammals, the replication checkpoint inhibits both origin firing and replication fork movement. To find out which strategy is employed in the fission yeast, Schizosaccharomyces pombe, we use...

journal_title：BMC molecular biology

authors： Mickle KL,Ramanathan S,Rosebrock A,Oliva A,Chaudari A,Yompakdee C,Scott D,Leatherwood J,Huberman JA

更新日期：2007-12-19 00:00:00

abstract：BACKGROUND:The mRNA translation initiation region (TIR) comprises the initiator codon, Shine-Dalgarno (SD) sequence and translational enhancers. Probably the most abundant class of enhancers contains A/U-rich sequences. We have tested the influence of SD sequence length and the presence of enhancers on the efficiency o...

journal_title：BMC molecular biology

authors： Vimberg V,Tats A,Remm M,Tenson T

更新日期：2007-10-31 00:00:00

abstract：BACKGROUND:Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme that plays critical functions in many biological processes, including DNA repair and gene transcription. The main function of PARP-1 is to catalyze the transfer of ADP-ribose units from nicotinamide adenine dinucleotide (NAD+) to a large array of acc...

journal_title：BMC molecular biology

authors： Zaniolo K,Desnoyers S,Leclerc S,Guérin SL

更新日期：2007-10-25 00:00:00

abstract：BACKGROUND:Although staphylokianse (SAK) is among the most promising blood dissolving agents, it is far from ideal. It is interesting to hypothesize that the clot lysis efficacy of SAK can be enhanced with direct active platelet binding ability, and at the same time the rethrombosis complication after successful recana...

journal_title：BMC molecular biology

authors： Chen H,Mo W,Su H,Zhang Y,Song H

更新日期：2007-10-07 00:00:00

abstract：BACKGROUND:We reported previously that the cardiac troponin I (cTnI) promoter drives cardiac-specific expression of reporter genes in cardiac muscle cells and in transgenic mice, and that disruption of GATA elements inactivates the cTnI promoter in cultured cardiomyocytes. We have now examined the role of cTnI promoter...

journal_title：BMC molecular biology

authors： Di Lisi R,Picard A,Ausoni S,Schiaffino S

更新日期：2007-09-17 00:00:00

abstract：BACKGROUND:Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. It is a key component of the viral RNA replicase complex that presumably includes other viral nonstructural and cellular proteins. The biochemical proper...

journal_title：BMC molecular biology

authors： Kim YG,Yoo JS,Kim JH,Kim CM,Oh JW

更新日期：2007-07-11 00:00:00

abstract：BACKGROUND:Physiological long term adaptation of pancreatic beta cells is driven by stimuli such as glucose and incretin hormones acting via cAMP (e.g. GLP-1) and involves regulated gene expression. Several rapidly inducible immediate-early genes (IEGs) have been identified in beta cells. Many of these IEGs code for tr...

journal_title：BMC molecular biology

authors： Glauser DA,Brun T,Gauthier BR,Schlegel W

更新日期：2007-06-22 00:00:00

abstract：BACKGROUND:Previous studies suggest that human RAD9 (hRad9), encoding a DNA damage checkpoint molecule, which is frequently amplified in epithelial tumor cells of breast, lung, head and neck cancer, participates in regulation of the tumor suppressor p53-dependent transactivation of pro-survival P21WAF1. This study exam...

journal_title：BMC molecular biology

authors： Ishikawa K,Ishii H,Murakumo Y,Mimori K,Kobayashi M,Yamamoto K,Mori M,Nishino H,Furukawa Y,Ichimura K

更新日期：2007-05-21 00:00:00

abstract：BACKGROUND:Initiation of eukaryotic DNA replication involves many protein-protein and protein-DNA interactions. We have previously shown that 14-3-3 proteins bind cruciform DNA and associate with mammalian and yeast replication origins in a cell cycle dependent manner. RESULTS:By expressing the human 14-3-3epsilon, as...

journal_title：BMC molecular biology

authors： Yahyaoui W,Callejo M,Price GB,Zannis-Hadjopoulos M

更新日期：2007-04-12 00:00:00

abstract：BACKGROUND:Invitrogen Gateway technology exploits the integrase/att site-specific recombination system for directional cloning of PCR products and the subsequent subcloning into destination vectors. One or three DNA segments can be cloned using Gateway or MultiSite Gateway respectively. A vast number of single-site Gat...

journal_title：BMC molecular biology

authors： Magnani E,Bartling L,Hake S

更新日期：2006-12-06 00:00:00

abstract：BACKGROUND:Macrophages (Mtheta) play a central role in the innate immune response and in the pathology of chronic inflammatory diseases. Macrophages treated with Th2-type cytokines such as Interleukin-4 (IL-4) and Interleukin-13 (IL-13) exhibit an altered phenotype and such alternatively activated macrophages are impor...

journal_title：BMC molecular biology

authors： Liddiard K,Welch JS,Lozach J,Heinz S,Glass CK,Greaves DR

更新日期：2006-11-29 00:00:00

abstract：BACKGROUND:Visceral leishmaniasis caused by members of the Leishmania donovani complex is often fatal in the absence of treatment. Research has been hampered by the lack of good laboratory models and tools for genetic manipulation. In this study, we have characterised a L. infantum line (JPCM5) that was isolated from a...

journal_title：BMC molecular biology

authors： Denise H,Poot J,Jiménez M,Ambit A,Herrmann DC,Vermeulen AN,Coombs GH,Mottram JC

更新日期：2006-11-13 00:00:00

abstract：BACKGROUND:Naturally occurring tRNAs contain numerous modified nucleosides. They are formed by enzymatic modification of the primary transcripts during the complex RNA maturation process. In model organisms Escherichia coli and Saccharomyces cerevisiae most enzymes involved in this process have been identified. Interes...

journal_title：BMC molecular biology

authors： Purta E,van Vliet F,Tkaczuk KL,Dunin-Horkawicz S,Mori H,Droogmans L,Bujnicki JM

更新日期：2006-07-18 00:00:00

abstract：BACKGROUND:Cdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease. RESULTS:Insertion of a TEV protease cleavage site into Cdc23 allows i...

journal_title：BMC molecular biology

authors： Yang X,Gregan J,Lindner K,Young H,Kearsey SE

更新日期：2005-06-07 00:00:00

abstract：BACKGROUND:When estimating relative transcript abundances by quantitative real-time PCR (Q-PCR) we found that the results can vary dramatically depending on the method chosen for data analysis. RESULTS:Analyses of Q-PCR results from a salmon louse starvation experiment show that, even with apparently good raw data, di...

journal_title：BMC molecular biology

authors： Skern R,Frost P,Nilsen F

更新日期：2005-04-26 00:00:00

abstract：BACKGROUND:Trichomonosis, caused by Trichomonas vaginalis, is the number one, nonviral sexually transmitted infection that has adverse consequences for the health of women and children. The interaction of T. vaginalis with vaginal epithelial cells (VECs), a step preparatory to infection, is mediated in part by the prom...

journal_title：BMC molecular biology

authors： Kucknoor AS,Mundodi V,Alderete JF

更新日期：2005-03-04 00:00:00

abstract：BACKGROUND:The enzyme glucose-6-phosphatase catalyzes the dephosphorylation of glucose-6-phosphatase to glucose, the final step in the gluconeogenic and glycogenolytic pathways. Expression of the glucose-6-phosphatase gene is induced by glucocorticoids and elevated levels of intracellular cAMP. The effect of cAMP in re...

journal_title：BMC molecular biology

authors： Thiel G,Al Sarraj J,Stefano L

更新日期：2005-01-19 00:00:00

abstract：BACKGROUND:Sequence periodicity with a period close to the DNA helical repeat is a very basic genomic property. This genomic feature was demonstrated for many prokaryotic genomes. The Escherichia coli sequences display the period close to 11 base pairs. RESULTS:Here we demonstrate that practically only ApA/TpT dinucle...

journal_title：BMC molecular biology

authors： Hosid S,Trifonov EN,Bolshoy A

更新日期：2004-08-26 00:00:00

abstract：BACKGROUND:Although the complete genome sequences of a large number of organisms have been determined, the exact proteomes need to be characterized. More specifically, the extent to which post-translational processes such as proteolysis affect the synthesized proteins has remained unappreciated. We examined this issue ...

journal_title：BMC molecular biology

authors： Kumar R,Musiyenko A,Oldenburg A,Adams B,Barik S

更新日期：2004-07-01 00:00:00

abstract：BACKGROUND:Double-strand breakage of chromosomal DNA is obviously a serious threat to cells because various activities of the chromosome depend on its integrity. However, recent experiments suggest that such breakage may occur frequently during "normal" growth in various organisms - from bacteria through vertebrates, p...

journal_title：BMC molecular biology

authors： Handa N,Kobayashi I

更新日期：2003-04-28 00:00:00

abstract：BACKGROUND:Cyclic AMP-dependent protein kinase (PKA) plays a central role in regulation of energy metabolism. Upon stimulation of testicular Sertoli cells by follicle stimulating hormone (FSH), glycolysis is activated to increase the production of nutrients for the germ cells, and a new regulatory subunit of cAMP-depen...

journal_title：BMC molecular biology

authors： Dahle MK,Taskén K,Taskén KA

更新日期：2002-06-21 00:00:00

abstract：BACKGROUND:cDNA-RDA is one of the subtractive cloning techniques used to isolate differentially expressed genes between two complex cDNA populations. In the present study we present a modification of the protocol described by Hubank and Schatz. RESULTS:In the post-hybridization mix, the 5'-ends of homoduplexes of inte...

journal_title：BMC molecular biology

authors： Kuvbachieva AA,Goffinet AM

更新日期：2002-04-24 00:00:00

abstract：BACKGROUND:Germline mutations in the tumor suppressor PTEN predispose human beings to breast cancer, and genetic and epigenetic alterations of PTEN are also detected in sporadic human breast cancer. Germline Pten mutations in mice lead to the development of a variety of tumors, but mammary carcinomas are infrequently f...

journal_title：BMC molecular biology

authors： Li Y,Podsypanina K,Liu X,Crane A,Tan LK,Parsons R,Varmus HE

更新日期：2001-01-01 00:00:00